#Gapdh western blot plus

#Gapdh western blot plus

Remarks: GAPDH Western blot 5-minute set contains monoclonal mouse anti-GAPDH, MAb 6C5, horseradish-peroxidase conjugated plus human GAPDH antigen as internal standard/calibator. The first step in a western blotting procedure is to separate the macromolecules in a sample using gel electrophoresis. Our Anti-GAPDH monoclonal antibodies are well characterized with MAb 6C5 suitable for GAPDH immunodetection in Western blotting, sandwich immunoassay and immunocytochemical applications. Some physiological factors, such as hypoxia and diabetes, can increase GAPDH expression in certain cell types. 6C5, are suitable for GAPDH immunodetection in. It is also useful for cell visualizing in microscopy assays. Anti-GAPDH monoclonal antibodies developed by HyTest, especially the well characterized MAb. Since it is constitutively and stably expressed in almost all tissues at a high level, GAPDH has become a well-established “housekeeping” protein and is widely used as a loading control for protein normalization in such procedures as Western blotting. GAPDH is highly conserved across species. Anti-Myc and GAPDH monoclonal antibodies were purchased from Santa Cruz Biotechnology. The GAPDH molecule is a homotetramer composed of four 36 kDa subunits. GAPDH is a housekeeping gene and used as controls in both Western blot and qPCR. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and. (For GAPDH immunodetection, see protocol page 3 AssayNotes below.)Ĭlick here: GAPDH AssayNotes and protocol. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation.

Besides functioning as a glycolytic enzyme in the cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, and DNA replication and repair.ĭuring the last decades many findings have been made concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death and age-related neuronal diseases such as Alzheimer’s and Huntington’s diseases. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is one of the key enzymes involved in glycolysis, catalyzing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate. Here we announce a direct MAb 6C5-HRP conjugate in place of secondary antibodies plus a GAPDH loading control for Western blots and protein normalization available online.